GenePharma offers state-of-the-art miRNA technology: Agomir and Antagomir. These specially labeled and chemically-modified microRNAs can be viewed as an upgraded product of miRNA mimics and inhibitors. Agomir is double-stranded and can regulate the biological function of the target gene by mimicking endogenous microRNAs. Antagomir, with a design that is based on the mature microRNA sequence, is single-stranded and is specially developed for inhibiting the expression of endogenous microRNA.

The Advantages of Antagomir and Agomir:

• Compared with common miRNA inhibitors and mimics, both Antagomir and Agomir have a higher affinity for the cell membrane. Therefore, the amount of transfection reagent could be drastically reduced.
• Especially suitable for in vivo RNAi experiments in live animals because of their higher stability and inhibiting effect. Furthermore, they can be injected into the animal by method of intravenous or local injection.
• They can be enriched in target cells, inducing effective, specific and stable interfering effects.
• The interfering effect can last a long time, at least one week; the longest interfering effect can be up to 5-6 weeks.

The Chemically-modified Construction of Antagomir and Agomir
Antagomir: 3’-terminal is modified by cholesterol, there are two thiol modifications in the 5’-terminal, four thiol modifications in the 3’-terminal, and the whole strand is modified by 2’-OMe.
Agomir: in the antisense strand, 3’ terminal is modified by cholesterol, there are two thiol modifications in the 5’-terminal, four thiol modifications in the 3’-terminal, and the whole strand is modified by OMe.

Comparison of experimental results between Antagomir and other inhibiting miRNAs
 

(Fig. a) shows the interfering effect of antagomir miRNA is much better than other chemical modified miRNA.
(Fig. b) Antagomir can inhibit the target microRNA specifically. The effects of antagomir -122 were found to be specific because a control for antagomir -122 that harbored four mismatch mutations (mm- antagomir-122) had no effect on miR-122 levels. Furthermore, expression levels of let7 and miR-22 were unaffected treated with antagomir-122 and mm-antagomir-122, indicating that silencing was miRNA-specific.

For technical questions, email support@genepharma.com. For questions related to order placement, email bd@genepharma.com.