CRISPR-based Genome editing technology

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a microbial nuclease system involved in the defense against invading phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR associated (Cas) genes, as well as non-coding RNA elements capable of programming the specificity of the CRISPR-mediated nucleic acid cleavage. Using the CRISPR/Cas 9 system with tens of thousands of guides that are designed to target all coding genes in the human genome allows researchers to conduct genome-scale gain- and loss-of function genetic screens.

One-step Generation of Mice with Multiple Mutations

Surveyor Assay