miRNA RT-PCR Detection Kits

Hairpin-itTM miRNAs qPCR Quantitation Kit
Genepharma’s Hairpin-itTM miRNAs qPCR Quantitation Kit is a sensitive and specific method using real-time PCR for the detection and quantification of micro RNAs (miRNA) from total RNA samples. MiRNAs are small, single-stranded, ~19–23 nucleotide RNA molecules encoded in the genomes of plants, animals, and viruses that play an important role in the control of gene expression at the messenger RNA (mRNA) level. Mature miRNAs enter the RNA-induced silencing complex (RISC) and guide the RISC to induce translational repression or endonucleolytic cleavage of specific target mRNAs. Compared to commonly used methods for detection of miRNAs, the Hairpin-itTM miRNAs qPCR Quantitation Kit is more rapid and sensitive. 
The Kit contains a miRNA-specific RT and PCR primer set, and also includes a Molecular Beacon probe. The special hairpin-like miRNA RT primer and the miRNA-specific Beacon probe ensure the RT and PCR reaction would not be interfered by miRNA precursors. Therefore, the reaction template could be total RNA or even cell lysates.
To normalize for RNA content among different experimental samples, the U6 snRNA qPCR Normalization Kit (Cat # QPM-050) and 5S rRNA qPCR Normalization Kit (Cat # QPM-060) are available separately.
The Hairpin-itTM miRNAs qPCR Quantitation Kit Reagents box includes the following items. Store the components at –20℃. Reagents must be stored in the dark.

Accessory Products
Some of the reagents supplied in the Hairpin-itTM miRNAs qPCR Quantitation Kit as well as other products suitable for use with the kit are available separately from GenePharma. Ordering information is provided below.

 
For technical assistance, email support@genepharma.com. For questions related to order placement, email bd@genepharma.com.