shRNA Vectors

When a validated siRNA sequence (for example, obtained by a large-scale siRNA validation screening) needs to maintain gene silencing for a longer period of time, we recommend the use of a short-hairpin RNA (shRNA) vector system. A shRNA expression vector with an incorporated antibiotic resistance gene can: 1) continuously produce shRNAs inside the cell, and 2) allow for selection of transfected cells. GenePharma is committed to providing the most advanced and convenient shRNA related tools. We recently introduced a new generation highly efficient, ready to use shRNA expression plasmids: our SuperSilencing shRNA Expression Vector System achieves excellent suppression of the target gene, lasting for several weeks or longer.

GenePharma SuperSilencing shRNA Expression Vector characteristics:

• Stable shRNA expression to achieve continuous RNAi.
• Screening and selectable markers help to establish a stably transfected cell line.  
• Two cloning restriction sites: BamH I and BbsI. BbsI is a special restriction endonuclease that produces asymmetrical complementary sticky ends to ensure correct insertion of the target DNA oligo, and prevents self-circularization of the plasmid vector.
• Convenient and easy to operate.
• Economical: one only needs to synthesize DNA oligos against the target gene.

GenePharma can construct ten kinds of shRNA expression vectors, with or without selectable markers, based on two different plasmids (pGPH1 and pGPU6, see “instructions” and our Catalog for more details on the expression vectors):

pGPU6, pGPH1, pGPU6/Neo, pGPH1/Neo, pGPU6/Hygro, pGPH1/Hygro, pGPU6/GFP/Neo, pGPH1/GFP/Neo, pGPU6/RFP/Neo, pGPH1/RFP/Neo;

pGPU6, pGPH1: shRNA expression plasmids; Selectable and screening markers:

• Neo: Neomycin (Kan/G418) resistance gene
• Hygro: Hygromycin B resistance gene
• GFP: GFP reporter gene
• RFP: RFP reporter gene

GenePharma SuperSilencing shRNA Expression Vector Set
Each set contains 4 linear or cyclic shRNA expression vectors designed for stable RNAi knockdown of your target gene: we guarantee that at least one of them causes at least 70% inhibition efficiency at the mRNA level. Each set also includes the corresponding negative control vector and a GFP positive control vector. Additionally, we provide you with the overall solution.

For technical assistance, email support@genepharma.com. For questions related to order placement, email bd@genepharma.com.